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Name:
Designation:
Areas of Specialization:
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Nirmalya Ganguli
Scientist-C
Transgenesis, Animal Bioreactor, Direct Mammary Gland Transfection, Protein Engineering
nganguli[at]niab[dot]org[dot]in |
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Education and training:
Dr. Nirmalya Ganguli received his PhD (2015) in the field of Animal Biotechnology, from Department of Biochemistry, University of Delhi South Campus, Delhi. He did his Research Associateship on development of methods for testicular transgenesis and development of animal bioreactor at National Institute of Immunology, New Delhi from 2015 until he joined the National Institute of Animal Biotechnology in February 2017.
Research experience and contributions:
Dr. Ganguli has experience in the generation of transgenic mice, generation of gene knockdown mice using shRNA, production of therapeutic protein in milk, and testicular germ cell depletion & stem cell transplantation work.
His current research interests include development of easy method of transgenesis of farmed animals. This will help in generation of farm animal models of diseases and development of disease resistance and/or high yielding animal herd. He is working on development of non-GMO animal bioreactor by directly transfecting the epithelial cells of udder gland for production of animal/human therapeutic proteins in milk. He is standardising the testicular germ cell depletion and spermatogonial stem cell transplantation study in farm animals to aid in utilisation of non-productive bull for production of elite bull sperm. This will also help in production of transgenic sperm by specific and precise genomic modification (site specific integration or deletion). Along with this he also intends to investigate the genomic basis of udder gland development in farm animals.
Selected publications:
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A Usmani*, N Ganguli, SK Jain, N Ganguli, RK Sarkar, M Choubey, M Shukla, H Sarkar, SS Majumdar. 2016. Robust generation of transgenic mice by simple hypotonic solution mediated delivery of transgene in testicular germ cells. Molecular Therapy - Methods & Clinical Development 3:16076
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N Ganguli, N Wadhwa, A Usmani, N Kunj, N Ganguli, RK Sarkar, SM Ghorai, SS Majumdar. 2016. An efficient method for generating a germ cell depleted animal model for studies related to spermatogonial stem cell transplantation. Stem Cell Research and Therapy 7:142
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N Ganguli, N Ganguli, A Usmani, SS Majumdar. 2015. Isolation and functional characterization of buffalo (Bubalus bubalis) β-casein promoter for driving mammary epithelial cell-specific gene expression. Journal of Biotechnolog53-9
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A Usmani, N Ganguli, H Sarkar, S Dhup, SR Batta, M Vimal, N Ganguli, S Basu, P Nagarajan, SS Majumdar. 2013. A non-surgical approach for male germ cell mediated gene transmission through
transgenesis. Scientific Reports 3:3430
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